某铅锌矿的三维地质模型及储量估算

针对矿山现有的地质信息数据等，建立了以利用Surpac软件的三维地表模型、矿体模型及地质数据库，对矿山资源储量进行计算。基于地质统计学的理论，建立某铅锌矿地表模型、钻孔数据库、矿体模型、块体模型，对矿体内样品进行组合分析统计，采用幂次距离反比法对块体品位进行估值，最后根据国内对地质矿产资源的分类方法，按照不同边界品位、工业品位等分类依据，得出资源储量核实报告，为铅锌矿的后续生产，提供了可靠保证。结果表明：通过构建该矿地质数据库，建立的三维模型，可对地质工程、采矿工程、储量估算、矿石品位动态管理等提供技术支持。     

Microbial Upgrading of Acetate into Value-Added Products—Examining Microbial Diversity,Bioenergetic Constraints and Metabolic Engineering Approaches

Ecological concerns have recently led to the increasing trend to upgrade carbon contained in waste streams into valuable chemicals. One of these components is acetate. Its microbial upgrading is possible in various species, with Escherichia coli being the best-studied. Several chemicals derived from acetate have already been successfully produced in E. coli on a laboratory scale, including acetone, itaconic acid, mevalonate, and tyrosine. As acetate is a carbon source with a low energy content compared to glucose or glycerol, energy- and redox-balancing plays an important role in acetate-based growth and production. In addition to the energetic challenges, acetate has an inhibitory effect on microorganisms, reducing growth rates, and limiting product concentrations. Moreover, extensive metabolic engineering is necessary to obtain a broad range of acetate-based products. In this review, we illustrate some of the necessary energetic considerations to establish robust production processes by presenting calculations of maximum theoretical product and carbon yields. Moreover, different strategies to deal with energetic and metabolic challenges are presented. Finally, we summarize ways to alleviate acetate toxicity and give an overview of process engineering measures that enable sustainable acetate-based production of value-added chemicals.     

数据信息化时代下的工程造价管理

在经济不断迅猛发展的形势下,新时代的经济形式催生出一些多元化企业和职业,信息技术的不断发展使数据信息成为最具价值的财富。在信息传播极其迅速的今天,工程造价在数据信息时代下的管理模式也不断更新变化,以适应多元化的工程项目形式。因此信息数据的大量搜集和相关研究受到了各大科研机构和各类企业的高度关注。     

One-Step Rapid Fabrication of Cell-Only Living Fibers

Cellular aggregates are used as relevant regenerative building blocks, tissue models, and cell delivery platforms. Biomaterial-free structures are often assembled either as 2D cell sheets or spherical microaggregates, both incompatible with free-form deposition, and dependent on challenging processes for macroscale 3D upscaling. The continuous and elongated nature of fiber-shaped materials enables their deposition in unrestricted multiple directions. Cellular fiber fabrication has often required exogenously provided support proteins and/or the use of biomaterial-based sacrificial templates. Here, the rapid (<24 h) assembly of fiberoids is reported: living centimeter-long scaffold-free fibers of cells produced in the absence of exogenous materials or supplements. Adipose-derived mesenchymal stem cell fiberoids can be easily modulated into complex multidimensional geometries and show tissue-invasive properties while keeping the secretion of trophic factors. Proangiogenic properties studied on a chick chorioallantoic membrane in an ovo model are observed for heterotypic fiberoids containing endothelial cells. These micro-to-macrotissues may find application as morphogenic therapeutic and tissue-mimetic building blocks, with the ability to integrate 3D and 4D full biological materials.     

变分布的量子行为粒子群优化算法求解工程约束优化问题

针对工程形状设计领域中带有多个约束条件的非线性设计优化问题,提出了一种自适应的基于高斯分布的量子行为粒子群优化(AG-QPSO)算法。通过自适应地调整高斯分布,AG-QPSO算法能够在搜索的初始阶段有很强的全局搜索能力,随着搜索过程的进行,算法的局部搜索能力逐渐增强,从而满足了算法在搜索过程不同阶段的需要。为了验证算法的有效性,在压力容器和张弦设计问题这两个工程约束优化问题上进行50轮独立实验。实验结果表明,在满足所有约束条件的情况下,AG-QPSO算法在压力容器设计问题上取得了5890.9315的平均解和5885.3328的最优解,在张弦设计问题上取得了0.01096的平均解和0.01096的最优解,远优于标准粒子群优化(PSO)算法、具有量子行为的粒子群优化(QPSO)算法和高斯量子行为粒子群(G-QPSO)算法等现有的算法的结果,同时AG-QPSO算法取得的结果的方差较小,说明该算法具有很好的鲁棒性。     

Intersecting Xenobiology and Neometabolism To Bring Novel Chemistries to Life

The diversity of life relies on a handful of chemical elements (carbon, oxygen, hydrogen, nitrogen, sulfur and phosphorus) as part of essential building blocks; some other atoms are needed to a lesser extent, but most of the remaining elements are excluded from biology. This circumstance limits the scope of biochemical reactions in extant metabolism – yet it offers a phenomenal playground for synthetic biology. Xenobiology aims to bring novel bricks to life that could be exploited for (xeno)metabolite synthesis. In particular, the assembly of novel pathways engineered to handle nonbiological elements (neometabolism) will broaden chemical space beyond the reach of natural evolution. In this review, xeno-elements that could be blended into nature's biosynthetic portfolio are discussed together with their physicochemical properties and tools and strategies to incorporate them into biochemistry. We argue that current bioproduction methods can be revolutionized by bridging xenobiology and neometabolism for the synthesis of new-to-nature molecules, such as organohalides.     

Nonribosomal Peptides Produced by Minimal and Engineered Synthetases with Terminal Reductase Domains

Nonribosomal peptide synthetases (NRPSs) use terminal reductase domains for 2-electron reduction of the enzyme-bound thioester releasing the generated peptides as C-terminal aldehydes. Herein, we reveal the biosynthesis of a pyrazine that originates from an aldehyde-generating minimal NRPS termed ATRed in entomopathogenic Xenorhabdus indica. Reductase domains were also investigated in terms of NRPS engineering and, although no general applicable approach was deduced, we show that they can indeed be used for the production of similar natural and unnatural pyrazinones.     

Scavenging Bacterial Siderophores with Engineered Lipocalin Proteins as an Alternative Antimicrobial Strategy

Iron acquisition mediated by siderophores, high-affinity chelators for which bacteria have evolved specific synthesis and uptake mechanisms, plays a crucial role in microbiology and in host–pathogen interactions. In the ongoing fight against bacterial infections, this area has attracted biomedical interest. Beyond several approaches to interfere with siderophore-mediated iron uptake from medicinal and immunochemistry, the development of high-affinity protein scavengers that tightly complex the siderophores produced by pathogenic bacteria has appeared as a novel strategy. Such binding proteins have been engineered based on siderocalin—also known as lipocalin 2—an endogenous human scavenger of enterobactin and bacillibactin that controls the systemic spreading of commensal bacteria such as Escherichia coli. By using combinatorial protein design, siderocalin was reshaped to bind several siderophores from Pseudomonas aeruginosa and, in particular, petrobactin from Bacillus anthracis, none of which is recognized by the natural protein. Such engineered versions of siderocalin effectively suppress the growth of corresponding pathogenic bacteria by depriving them of their iron supply and offer the potential to complement antibiotic therapy in situations of acute or persistent infection.     

A minimalist approach to polarization resistance measurements in a reinforced concrete structure

In the curriculum of future civil engineers, electrochemical concepts are often neglected, even though this knowledge is essential to adequately maintain reinforced concrete structures in a chloride-exposed environment. Experiments are necessary to consolidate key theoretical concepts, but laboratory experience often involves complex and expensive electronic equipment that may obscure the basic procedures at work. This paper presents a simple minimalistic electrochemical test for polarization resistance measurements by which students can learn basic concepts of corrosion electrochemistry and estimate the corrosion rate as a key parameter for estimation of the remaining service life of concrete structures. The focus is on avoiding unnecessary complications and providing an experiment that can be readily implemented for a wider student reach.